Incorporation of 3-deoxy-3-fluoro-D-glucose into glycogen and trehalose in fat body and flight muscle in Locusta migratoria.
نویسندگان
چکیده
Flight muscle and fat body extracts from Locusta migratoria were incubated with D-[U-14C]-glucose or D-[3-3H]-3-deoxy-3-fluoroglucose and the products were analyzed. In the case of the latter compound, radio-chromatographic analysis yielded glycogen and trehalose fractions that were shown by 19F nuclear magnetic resonance to contain fluorine. Acid hydrolysis of these fractions liberated tritium labelled 3-deoxy-3-fluoro-D-glucose. In addition to the formation of "fluoroglycogen" and "fluorotrehalose" in these tissue extracts, there was an accumulation of tritium labelled fructose.
منابع مشابه
Changes in energy reserves during flight of the desert locust, Schistocerca gregaria.
-1. Amounts of glycogen and glucose in locust haemolymph and fat body increase during the first 15 rain of flight but later fall to below the resting values. 2. Amounts of trehalose in haemolymph and glycogen in gut wall decline throughout flight. 3. Variations in the concentrations of free amino acids in haemolymph are reported. 4. The concentration of lipid in the haemolymph increases to abou...
متن کاملIncorporation of amino acids into mitochondrial protein of the flight muscle of Locusta migratoria in vitro and in vivo in the presence of cycloheximide.
The incorporation of arnino acids into distinct protein fractions of mitochondria from various sources [1-4) including a cytoplasmic mutant of Neurospora crassa [5] has been shown tobe an inherent Capacity of isolated mitochondria in vitro. An independent method for studying this system is affered by the specific inhibition of extramitochondrial protein synthesis in vivo. Cycloheximide is known...
متن کاملEnergy substrates for flight in the blister beetle Decapotoma lunata (Meloidae)
We investigated the substrates for flight in the blister beetle Decapotoma lunata by (a) establishing the patterns of maximum activities of enzymes of various metabolic pathways in the flight muscles, (b) measuring the respiratory rates of flight muscle mitochondria with various substrates and (c) determining metabolite concentrations in flight muscles and haemolymph during a flight period of u...
متن کامل[Activity and subcellular localization of the enzyme adenyl cyclase in the flight-muscle of Locusta migratoria (author's transl)].
Wanderheuschrecken (Locusta migratoria) veratmen während eines längeren Fluges in den ersten dreißig Minuten vor allem Kohlenhydrate, später Fette1. In ihren Flugmuskeln können sowohl die Enzyme des Fettals auch die des Kohlenhydratabbaues nachgewiesen werden. Außerdem sinkt wäh rend des Fluges ihr Gehalt an Glykogen, Trehalose und Glukose2. Humorale, vielleicht auch nervöse Faktoren dürften d...
متن کاملOn the Release of the Three Locust (Locusta migratoria) Adipokinetic Hormones: Effect of Crustacean Cardioactive Peptide and Inhibition by Sugars
A n existing test to m onitor the rate of adipokinetic horm one release from the corpora cardiaca (CC) of Locusta migratoria in vitro was improved, so that a constant basal ra te of release was achieved and the am ount of released Lom -AKH-I, II and III could be quantified by HPLC. This test system was subsequently used to dem onstrate that a small peptide, which has been found in a few insect ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Bioscience reports
دوره 6 3 شماره
صفحات -
تاریخ انتشار 1986